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Type:	fermentor BIOSTAT Bplus 10L Twin 230 V	Soort:	bioreactor
Merk:	sartorius	Beschikbaar:	ja
Techniek:	Fermentatie
Instelling:	KU Leuven, Technology Campus Ghent
Locatie:	KU Leuven, Technology Campus Ghent; Process Unit Enzyme and Fermentation technology	Contact:	Ilse Van de Voorde
	Gebroeders De Smetstraat 1; 9000 Ghent		+32 9 265 86 10
	Belgium		ilse.vandevoorde@kuleuven.be

Technische Specificaties

Sterilisable stirred tank bioreactors with full process control capabilities (pH, temperature, aeration, dissolved oxygen concentration) for research, process development and scale-up using batch, fed-batch or continuous mode including high-cell density fermentation

Profiling of growth through analysis of optical density, dry cell weight, carbon source concentration and cell counting

The BIOSTAT Bplus has an integrated Gas Flow Ratio Control, and two integrated mass flow controllers for air and oxygen.

It is controlled via DO control loop which allows advanced process control and easy gas balancing.

The GFRC strategy enables highest oxygen transfer for high-cell density cultures as well as for sheer stress sensitive gassing for filamentous organisms.

It combines two operation modes for advanced gassing control of Air and O2: constant flow: percentage alteration; constant ratio: alteration of flow rates.

Digital controller: graphical user interface with color display and touch screen operation; measurement and control for temperature, pH, DO, agitation, foam & level; multi-stage DO cascade control; 2x feed controller per vessel; gas flow ratio controller; level control via level probe; trend display for up to 6 process values

Gas flow ratio control gassing system: gas mixing of air and O2; mass-flow controllers for air and O2 controlled via DO controller

Pumps: up to 4 integrated pumps per side; configurable to substrate controller; up to 2 external feed pumps per side

Temperature system: powerful heater (1 kW); integrated controlled cooling water valve; circulation pump; temperature range 8 °C above cooling water up to 80 °C

Agitation system: speed range from 20 up to 2000 rpm

UniVessel Culture vessel: Jacketed culture vessel fully equipped with: probes for temperature, DO, pH, foam and level; stirrer shaft with single mechanical seal; Rushton impeller; baffle assembly; aeration tube with ring sparger, sterile filters and exhaust cooler; manual sampler with sampling pipe; removable bottle holder; addition bottles with stainless steel head piece and sterile filters; inoculation/addition port; four-way addition port

SCADA Software BioPAT MFCS/DA: online data acquisition; sample data management; export functions

Toepassings gebieden

Culture of microorganisms

Batch, fed batch and continuous culture

Small scale cell mass and protein production

High-cell density culture

Culture of filamentous microorganisms

Specific expertise: Production, application and characterization of a wide range of enzymes for the food and feed industry

Development of starter cultures for food (cheese, salami, yoghurt, …) and feed correlated aroma and probiotica

Production of enzymes (isomerases, cellulases, xylosidases, …) for improvement and development of processes in food and feed industry

Production of bio-ethanol including pretreatment of renewable raw materials and pentose and hexose fermentation

Microbiological conversion of side streams into value added products

Full process development of innovative food ingredients (alternative sweeteners, polyols, …) based on microbiological/bio-catalytic processes, including recovery and purification with high product quality

Food Pilot – op site ILVO eenheid Technologie en Voeding – Brusselsesteenweg 370 – 9090 Melle – België – T +32 9 272 30 00 – F +32 9 272 30 01